Melvin Fribourg.

 

About me

Serious, involved and rigorous in my work. Very comfortable in teamwork. Motivated and competent in the field of biotechnologies, with good analytical skills and good interpersonal skills. With strong technical and theoretical bases in cell biology, microbiology, genetic engineering, biochemistry and bioinformatics.

 

Diplomas and training

Frontiers of Life Sciences Bachelor's : 2nd year

2019 - 2020

This bachelor allowed me to learn and deepen my knowledge in life sciences, on the world of research and the role of science in society.
The program is based on a common core consisting of mathematics, physics, chemistry, computer science and biology in order to approach the living in all its facets.

Certificate of Higher Technician in Biotechnology

2017 - 2019

This two-year technical degree allowed me to obtain theoretical and technical knowledge in biotechnology, biochemistry and in laboratory manipulation.
The program of this two-year technical degree is based on a common core consisting of theoretical courses and practical work in molecular and genetic biology, cell biology, bioinformatics, biochemistry, microbiology, mathematics, physics, chemistry.

Baccalaureate STL Biotechnology series

2014  - 2017

This baccalaureate allowed me to obtain theoretical and technical knowledge in biotechnology, and in laboratory manipulation.
The program for this baccalaureate is based on a common core consisting of theoretical courses and practical work in biotechnology, biochemistry, mathematics, physics, chemistry.

 

Professional experiences

Internships as Senior Laboratory Technician

2018 - 2019

2 internships of 8 weeks in the laboratory of crystallography and biological NMR LCRB - UMR 8015 - CNRS University Paris Descartes Faculty of pharmaceutical and biological sciences 4 Avenue de l'Observatoire 75270 Paris Cedex 06.


Internships research theme was: “The initiation of translation” .


The main techniques that I used were: PCR, Transcription, Culture, Transformation, Electrophoresis (Agarose, SDS PAGE, Acrylamide Urea), Affinity columns.

Donor recruiter within the NGO Aide et Action

July 2019

Paris (1 month).
Missions:

  • Intercept passersby, and offer to support the association you represent with a regular donation.

Necessary and developed qualities:

  • Sensitivity to humanitarian, environmental or public health causes

  • Dynamic, enthusiastic, mobile and combative

  • Sense of contact and team spirit

Versatile Salesman at Primark

June 2019

Villeneuve-la-Garenne, Qwartz (1 month).
Missions:

  • Reassortment of shelves

  • Cleaning and maintenance of departments

  • Sales and advice to customers

Necessary and developed qualities:

  • Sense of presentation and courtesy

  • Welcoming, good interpersonal skills

  • Sense of service

  • Sense of organization

  • Product knowledge

Versatile Salesman at Action

August 2019

Villeneuve-la-Garenne, Qwartz (1 month).
Missions:

  • Reassortment of shelves

  • Cleaning and maintenance of departments

  • Sales and advice to customers

Necessary and developed qualities:

  • Sense of presentation and courtesy

  • Welcoming, good interpersonal skills

  • Sense of service

  • Sense of organization

  • Product knowledge

 

My skills

Molecular biology

  • DNA and RNA extraction and purification

  • Cell transformation

  • PCR

  • Electrophoresis

Cellular biology

  • Eukaryotic cell count

  • Culture of suspended or adherent eukaryotic cells

  • Practical study of the phases of mitosis

  • Cellular quantification by protein assay

  • Primary culture.

  • Cell viability measurement (trypan blue, DAF, MTT)

  • Immunocytochemistry (tubulin labeling)

  • Microscopy

Microbiology

  • Orientation and identification of microorganisms

  • Culture of microorganisms

  • Enumeration of microorganisms

  • Growth monitoring

  • Bioreactor

  • Antibiogram

Biochemistry

  • Spectrophotometric assay

  • Buffer solution and standard preparation

  • Chromatography

  • Elisa test

Computer science

  • Office Pack: Microsoft Word, Excel, Powerpoint

  • Bioinformatics: Navigation from the NCBI portal, NEBcutter, Serial Cloner, BLAST, Oligoanalyser, Benchling

  • Adobe: Photoshop, Lightroom

  • Programming language: Python, R, Shell

Interests

  • Basketball

  • Boxing

  • Music

  • Photography

  • Infographics

 
 

Projects

Some samples of my work

AQUATHON

During the Aquathon project, which lasted 2 weeks, we tested the seawater quality in Brest to determine the well being of Brest’s ecosystems.We planned to compare the water quality from multiple locations in Brest and set them against global ocean data.We wanted to test the accuracy of accessible water test kits on seawater.We also aspire to participate in the development of citizen science projects around water testing.

CHLAMYDOMONAS REINHARDTII

This project aims to present a model organism used in biology, in poster form. Here: Chlamydomonas reinhardtii